The use of PTFE filter elements in the biotechnological production of antibiotic substance

Abstract

The paper shows the prospects of further research on the use of porous filters based on fluoroplastic in the production of antibiotic subtancies on the example of pyocyanin.

The analysis of research has revealed the urgency of the problem of antibiotic resistance of infectious agents, in this regard, there is a need to develop new types of antibiotics or improve existing ones.

Today, the main method of obtaining new antibiotics is biosynthesis, which is carried out by fermentation of the corresponding microorganism, and the final stage of any biotechnological production is the isolation of the target product from the nutrient medium and its purification. The initial stage of product isolation is filtration using filters of various designs. Among the large number of filter designs and filter elements of various compositions offered on the market, we focused on fluoroplastic filter elements.

The antimicrobial activity of P. aeruginosa culture liquid by two methods - the method of perpendicular strokes and two-fold dilutions studied. The research results showed that the antimicrobial activity slightly increased after filtration, which may be due to the concentration of cells and, accordingly, the release of a larger amount of antimicrobial substances by the cells of the P. aeruginosa microorganism.