Investigation of the use of fluoroplastic filter elements in the production of a promising antibiotic substance pyocyanin

Abstract

The paper shows the prospects of further research on the use of porous filters based on fluoroplastic in the production of antibiotic subtancies on the example of pyocyanin.

The analysis of research has revealed the urgency of the problem of antibiotic resistance of infectious agents, in this regard, there is a need to develop new types of antibiotics or improve existing ones.

Today, the main method of obtaining new antibiotics is biosynthesis, which is carried out by fermentation of the corresponding microorganism, and the final stage of any biotechnological production is the isolation of the target product from the nutrient medium and its purification. The initial stage of product isolation is filtration using filters of various designs. Among the large number of filter designs and filter elements of various compositions offered on the market, we focused on fluoroplastic filter elements.

We determined the filtration efficiency of the culture fluid P. aeruginosa - a producer of the antibiotic substance pyocyanin through fluoroplastic filters, studied the permeability of the filter element and the influence of the filter element thickness on the filtration process. Separation of culture fluid of P. aeruginosa from biomass through fluoroplastic filters showed filtration efficiency. Carrying out series of filtrations using the same filter showed preservation of permeability of a filter element. The study of the influence of the thickness of the filter element on the filtration process showed an increase in the efficiency of filtration with increasing thickness.